The SampleStream™ Platform is here.

Intact Protein Analysis Transformed.

The heart of the platform is a fluidic module designed for extreme concentration of dilute samples, rapid buffer exchange from complex matrices, and ultra-robust, unattended, high-throughput operation.

Integrated with the industry gold-standard PAL 3 robot, the complete SampleStream Platform provides powerful workflow automation capabilities, all in a compact footprint and at a competitive price point.

Beautifully Simple Technology

The heart of the SampleStream platform is a small fluidic channel that incorporates a molecular weight cutoff membrane. Combined with bullet-proof and time tested syringe pumps and valves, this channel operates in one of two modes.

Liquid Chromatography, Replaced.

  • Low pressure operation (<30 bar)

  • Driven by low maintenance syringe pumps
  • Single membrane good for >1,000 analyses
  • High membrane area enables high wash volume in comparison to liquid chromatography

Sample Focusing

Focusing

Flows entering from two inlet ports cause solutes retained by the membrane to accumulate at the point of zero axial flow.

Solvent Insensitivity

Since nothing is ever bound to a stationary phase, the SampleStream module is insensitive to loading buffer conditions.

Concentration Factor

With the proper membrane selected, the fluidic channel becomes leak-tight for retained analytes, enabling concentration of analytes from extremely dilute samples in record time. In testing, we have achieved >1,000-fold enrichment of proteins.

Buffer Exchange

Pressure driven flow across the membrane enables a large volume of buffer to wash retained solutes in record time.

Sample Elution

Elution

Reverse flow across the membrane, with one inlet port blocked, lifts samples from the membrane and directs them out of the channel for downstream processing.

Reverse Flow

Reverse flow across the membrane lifts any adsorbed biomolecules off the membrane, ensuring efficient elution and low carryover. Further, membrane fouling is slowed, enabling a single membrane to function for >1,000 analyses.

Consistent Elution Volume

Regardless of input concentration, analytes are always eluted in the same volume, yielding predictable concentration enrichment.

Quantitative Performance

Linear quantitative performance over wide range of mass loading (10 ng to 1 ug).

High Performance Biomolecular Analysis

The SampleStream™ Platform has unique characteristics that give it surprising capability.

High-throughput operation.  As little as 15 sec/sample for mAbs in excipient. (Click to Enlarge)

Carryover measured at different loading amounts. With carryover routinely below 5%, throughput is further increase in routine screening modalities. (Click to Enlarge)

Comparison to traditional 2.1 mm chromatography. Time axis normalized. (Click to Enlarge)

Operation with native buffer. The SampleStream platform can operate in native mode on as little as 100 ng of material. Importantly, switching to native mode doesn’t affect throughput. (Click to Enlarge)

A calibration curve for Herceptin showing quantitative performance over a wide loading range. Coupled with throughput, the SampleStream Platform can radically change clinical proteomics. (Click to Enlarge)

Consistent peak volume independent of loading volume leads to high concentration factors at higher loading volumes. Feed it a large volume of dilute sample, get a concentrated sample zone. (Click to Enlarge)